兔抗EID1多克隆抗體

Background:

E1A-like inhibitor of differentiation-1 (EID-1), an acetyltransferase enzyme, binds both the retinoblastoma protein (Rb), a regulator of cell cycle and tissue specific transcription, and the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. EID-1 inhibits cellular differentiation by blocking the histone acetyltransferase activity of p300. EID-1 also acetylates both histones and non-histone proteins such as NCOA3 co-activator. By acetylating histones, EID-1 gives a specific tag for transcriptional activation. In addition to binding Rb and p300, EID-1 also binds to phosphorylated CREB protein, mediating cAMP gene regulation. EID-1 augments the activity of phosphorylated CREB and activates transcription of cAMP responsive genes as a co-activator.

Applications:

ELISA, WB, IHC

Name of antibody:

EID1

Immunogen:

Fusion protein of human EID1

Full name:

EP300 interacting inhibitor of differentiation 1

Synonyms：

CRI1; EID-1; RBP21; PTD014; C15orf3; PNAS-22; IRO45620

SwissProt:

Q9Y6B2

ELISA Recommended dilution:

1000-2000

IHC positive control:

Human colon cancer and human esophagus cancer

IHC Recommend dilution:

25-100

WB Predicted band size:

21 kDa

WB Positive control:

Mouse heart tissue

WB Recommended dilution:

200-1000