兔抗MRPL42多克隆抗體

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q.

Applications:

ELISA, WB, IHC

Name of antibody:

MRPL42

Immunogen:

Fusion protein of human MRPL42

Full name:

mitochondrial ribosomal protein L42

Synonyms:

L31MT; L42MT; S32MT; MRPL31; MRPS32; PTD007; RPML31; HSPC204; MRP-L31; MRP-L42; MRP-S32

SwissProt:

Q9Y6G3

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human cervical cancer and Human colorectal cancer

IHC Recommend dilution:

50-200

WB Predicted band size:

17 kDa

WB Positive control:

Jurkat, HepG2, Hela and Raji cell lysates

WB Recommended dilution:

1000-5000