兔抗ORC1多克隆抗體

Background:

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Applications:

ELISA, IHC

Name of antibody:

ORC1

Immunogen:

Fusion protein of human ORC1

Full name:

origin recognition complex subunit 1

Synonyms:

ORC1L; PARC1; HSORC1

SwissProt:

Q13415

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human esophagus cancer and Human liver cancer

IHC Recommend dilution:

50-300