熒光素酶

其他中文名稱 ： 熒光素酶(螢火蟲)；重組型酵素 

英文名稱 ： Luciferase firefly 

其他英文名稱 ： Luciferase from Photinus pyralis (firefly) 

C A S ： 61970-00-1

級 別 ： BR

product line ： BioUltra

Assay ： ≥98% (SDS-PAGE)

Mol wt ： mol wt 120 kDa (two non-identical subunits, each containing four free thiol groups, one of which is necessary for activity)

Protein ： 10-35% E1%/280

Measuring range ： ≤1 femtomole ATP sensitivity, (using 0.2 μg of luciferase and suitably sensitive liquid scintillation counters or luminometers)

Foreign activity ： ATPase ≤5 nanomolar units/mg protein；Nucleoside diphosphokinase ≤20 nanomolar units/mg protein

活力定義 ： One light unit produces a biometer peak height equivalent to 0.02 μCi of 14C in PPO/POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25 °C

產(chǎn)品描述 ： Two contaminant, ATP-consuming activities are assayed for in this product, ATPase and nucleoside diphosphokinase. These impurities are found to be less than 5 nanomolar units/mg protein and less than 20 nanomolar units/mg protein, respectively. 

Note : Prior to 1991, a unit of firefly luciferase activity was defined as that amount which will produce 1.0 nanomole of pyrophosphate per minute at pH 7.7, 25 °C, using a system containing 0.6 mM ATP and 0.1 mM D-luciferin. The former nanomolar unit is equivalent to approximately 1.3 x 106 light units

性 狀 ： 粉末。EC：1.13.12.7

用 途 ： 生化研究。

保 存 ： -20℃。保質(zhì)期1年