小鼠抗BrdU單克隆抗體

Background:

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to research the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information, for example, the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

Applications:

IHC, IF

Name of antibody:

BrdU

Immunogen:

Bromodeoxyuridine coupled to keyhole limpet hemocyanin.

Full name:

Bromodeoxyuridine

IHC positive control:

colon tissue from BrdU injected mouse and colon tissue from IDU injected mouse

IHC Recommend dilution:

30-150

IF Positive control:

HT-29 cells pulsed with 5-bromo-2'-deoxyuridine (BrdU)

IF Recommended dilution:

100-200