小鼠抗IdU單克隆抗體

Background:

Iododeoxyuridine (5-Iodo-2'-deoxyuridine, IdU) is a synthetic thymidine nucleoside analog. IdU, like BrdU thymidine analog, is used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and IdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for IdU are subsequently used to detect the incorporated IdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat.

Applications:

IHC, IF

Name of antibody:

IdU

Immunogen:

Iododeoxyuridine coupled to keyhole limpet hemocyanin.

Full name:

Iododeoxyuridine

IHC positive control:

colon tissue from BrdU injected mouse and colon tissue from IDU injected mouse andcolon tissue from CLDU injected mouse

IHC Recommend dilution:

30-150

IF Positive control:

HT-29 cells pulsed with 5-iodo-2'-deoxyuridine (IdU)

IF Recommended dilution:

100-200