小鼠抗SHARPIN單克隆抗體

Background:

Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis.

Applications:

WB

Name of antibody:

SHARPIN

Immunogen:

Fusion protein of human SHARPIN

Full name:

SHANK associated RH domain interactor

Synonyms:

SIPL1

SwissProt:

Q9H0F6

WB Predicted band size:

40 KD

WB Positive control:

Hela, Jurkat, 293T cell lysates

WB Recommended dilution:

500-2000