小鼠抗PRKAB1單克隆抗體

Background:

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.

Applications:

WB, IF, IP, IHC

Name of antibody:

PRKAB1

Immunogen:

Fusion protein of human PRKAB1

Full name:

protein kinase AMP-activated non-catalytic subunit beta 1

Synonyms:

AMPK; HAMPKb

SwissProt:

Q9Y478

IHC positive control:

Human breast cancer

IHC Recommend dilution:

100-200

WB Predicted band size:

30 kDa

WB Positive control:

3T3, Hela, PC-12, COS7 and MDA-MB-468 cell lysates

WB Recommended dilution:

1000-5000

IF positive control:

Hela cells

IF Recommend dilution:

50-100