中文名稱: SP20細(xì)胞裂解液
英文名稱: SP20 Cell Lysate
別 名: SP20細(xì)胞裂解液
相關(guān)類別:抗原
儲 存: 冷凍(-20℃) 避光
概 述
SP20 cell lysates from mouse myeloma cells were cultured in the media containing 10% FBS.
技術(shù)規(guī)格
Background |
Cells were lysed in lysis buffer (50mM Tris(PH7.4), 150mM NaCl, 1% NP-40, 1% SDS). 100 μg of cellular protein was mixed with 4 times volume of 5X SDS-PAGE loading buffer. |