技術(shù)規(guī)格
Background: |
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]. |
Applications: |
WB, IHC, IF |
Name of antibody: |
RFC4 |
Immunogen: |
Fusion protein of human RFC4 |
Full name: |
replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 1 |
Synonyms: |
A1; RFC37 |
SwissProt: |
P35249 |
IHC positive control: |
human pancreas tissue and carcinoma of human pancreas tissue; human tonsil tissue |
IHC Recommend dilution: |
30-150 |
WB Predicted band size: |
40 kDa |
WB Positive control: |
HepG2, Hela, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF-7 cell lysates |
WB Recommended dilution: |
500-2000 |